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rabbit polyclonal antisera against pelp1 mnar (Bethyl)

Name: rabbit polyclonal antisera against pelp1 mnar
Brand: Bethyl
Rating: 93 (47 reviews)

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Bethyl rabbit polyclonal antisera against pelp1 mnar
Rabbit Polyclonal Antisera Against Pelp1 Mnar, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal antisera against pelp1 mnar/product/Bethyl
Average 93 stars, based on 47 article reviews

rabbit polyclonal antisera against pelp1 mnar - by Bioz Stars, 2026-03

93/100 stars

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Journal: Frontiers in Oncology

Article Title: PELP1 Suppression Inhibits Gastric Cancer Through Downregulation of c-Src-PI3K-ERK Pathway

doi: 10.3389/fonc.2019.01423

Figure Lengend Snippet: Primers for Q-PCR.

Article Snippet: Immunohistochemical staining using a polyclonal antibody against PELP1 (A13414, 1:100 for IHC analysis, ABclonal) was performed.

Techniques:

PELP1 expression was elevated in gastric cancer tissues and cells comparing with corresponding counterparts. (A) Box plots derived from gene expression data in ONCOMINE comparing expression of a specific PELP1 in different cancer tissues. (B1–B4) Oncomine data showed that PELP1 expression was elevated in GC tissues as compared with gastric normal tissues. (C1) Representative images of PELP1 level in normal gastric mucosa and GC specimens by immunohistochemistry. The positive rate of PELP1 in gastric cancer specimens was higher than in normal gastric mucosa. (C2) , PELP1 expression in normal gastric and GC tissues. The mean density of cancerous and adjacent gastric tissues from all 36 cases is illustrated at top graph. Difference was detected between the mean density of cancerous tissues (0.0346 ± 0.0172) and that of adjacent tissue (0.0235 ± 0.0115) using paired students' test, p < 0.05. (D) PELP1 was expressed in different stomach cell lines from CCLE. (E1,E2) The level of PELP1, ERα and ERβ was induced from Oncomine data base and validated by western bot in four GC cell lines and nomal gastric epithelium cell line GES-1. Mean ± SEM was applied for analysis (* p < 0.05, *** p < 0.001).

Journal: Frontiers in Oncology

Article Title: PELP1 Suppression Inhibits Gastric Cancer Through Downregulation of c-Src-PI3K-ERK Pathway

doi: 10.3389/fonc.2019.01423

Figure Lengend Snippet: PELP1 expression was elevated in gastric cancer tissues and cells comparing with corresponding counterparts. (A) Box plots derived from gene expression data in ONCOMINE comparing expression of a specific PELP1 in different cancer tissues. (B1–B4) Oncomine data showed that PELP1 expression was elevated in GC tissues as compared with gastric normal tissues. (C1) Representative images of PELP1 level in normal gastric mucosa and GC specimens by immunohistochemistry. The positive rate of PELP1 in gastric cancer specimens was higher than in normal gastric mucosa. (C2) , PELP1 expression in normal gastric and GC tissues. The mean density of cancerous and adjacent gastric tissues from all 36 cases is illustrated at top graph. Difference was detected between the mean density of cancerous tissues (0.0346 ± 0.0172) and that of adjacent tissue (0.0235 ± 0.0115) using paired students' test, p < 0.05. (D) PELP1 was expressed in different stomach cell lines from CCLE. (E1,E2) The level of PELP1, ERα and ERβ was induced from Oncomine data base and validated by western bot in four GC cell lines and nomal gastric epithelium cell line GES-1. Mean ± SEM was applied for analysis (* p < 0.05, *** p < 0.001).

Article Snippet: Immunohistochemical staining using a polyclonal antibody against PELP1 (A13414, 1:100 for IHC analysis, ABclonal) was performed.

Techniques: Expressing, Derivative Assay, Gene Expression, Immunohistochemistry, Western Blot

Relationship between clinicopathological characteristic and PELP1 expression in gastric cancer.

Journal: Frontiers in Oncology

Article Title: PELP1 Suppression Inhibits Gastric Cancer Through Downregulation of c-Src-PI3K-ERK Pathway

doi: 10.3389/fonc.2019.01423

Figure Lengend Snippet: Relationship between clinicopathological characteristic and PELP1 expression in gastric cancer.

Article Snippet: Immunohistochemical staining using a polyclonal antibody against PELP1 (A13414, 1:100 for IHC analysis, ABclonal) was performed.

Techniques: Expressing

PELP1 knowdown inhibited proliferation, migration and invasion, xenograft of gastric cancer cell. (A) The absorbance (OD) at 450 nm wave-length was reduced in PELP1 knockdown group in CCK-8 assays. PELP1 silencing inhibited the colony formation ability of AGS (B1) and SNU-1 (B2) . (B3) Representative image for colony formation. (C1,C2) Representative image of the EdU assay confirmed that mean density was reduced in PELP1 knockdown group. (D) PELP1 knowdown prevented the cell progression from S phase into M phase in cell cycle arrest. The data represent results from one of three independent experiments. (E1,E2) Wound healing assay was used to detect the change of migration of cells. The migration ability of AGS was reduced in PELP1 knockdown group. Transwell small chamber assay was used to detect the change of migration and invasion of GC cells. (F1,F2) The migration and invasion ability of AGS was reduced in PELP1 knockdown group. (F3,F4) The migration and invasion ability of SNU-1 was reduced in PELP1-siRNA group. (G) PELP1 silencing inhibited the growth of xenograft in AGS cell inoculated nue mice. Experiments were repeated 3 or 4 times. Values are the mean ± SEM (* p < 0.05, ** p < 0.01, *** p < 0.001).

Journal: Frontiers in Oncology

Article Title: PELP1 Suppression Inhibits Gastric Cancer Through Downregulation of c-Src-PI3K-ERK Pathway

doi: 10.3389/fonc.2019.01423

Figure Lengend Snippet: PELP1 knowdown inhibited proliferation, migration and invasion, xenograft of gastric cancer cell. (A) The absorbance (OD) at 450 nm wave-length was reduced in PELP1 knockdown group in CCK-8 assays. PELP1 silencing inhibited the colony formation ability of AGS (B1) and SNU-1 (B2) . (B3) Representative image for colony formation. (C1,C2) Representative image of the EdU assay confirmed that mean density was reduced in PELP1 knockdown group. (D) PELP1 knowdown prevented the cell progression from S phase into M phase in cell cycle arrest. The data represent results from one of three independent experiments. (E1,E2) Wound healing assay was used to detect the change of migration of cells. The migration ability of AGS was reduced in PELP1 knockdown group. Transwell small chamber assay was used to detect the change of migration and invasion of GC cells. (F1,F2) The migration and invasion ability of AGS was reduced in PELP1 knockdown group. (F3,F4) The migration and invasion ability of SNU-1 was reduced in PELP1-siRNA group. (G) PELP1 silencing inhibited the growth of xenograft in AGS cell inoculated nue mice. Experiments were repeated 3 or 4 times. Values are the mean ± SEM (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Immunohistochemical staining using a polyclonal antibody against PELP1 (A13414, 1:100 for IHC analysis, ABclonal) was performed.

Techniques: Migration, Knockdown, CCK-8 Assay, EdU Assay, Wound Healing Assay, Boyden Chamber Assay

PELP1 activation promoted proliferation, colony formation, migration of GES-1 in vitro . (A) Representative image of western blot for testing the activation effect of saRNA to PELP1 in GES-1. (B) saRNA for PELP1 increased the proliferation ability of GES-1. (C) saRNA for PELP1 increased the colony formation ability of GES-1. (D) saRNA for PELP1 increased the invasion ability of GES-1. *** p < 0.001.

Journal: Frontiers in Oncology

Article Title: PELP1 Suppression Inhibits Gastric Cancer Through Downregulation of c-Src-PI3K-ERK Pathway

doi: 10.3389/fonc.2019.01423

Figure Lengend Snippet: PELP1 activation promoted proliferation, colony formation, migration of GES-1 in vitro . (A) Representative image of western blot for testing the activation effect of saRNA to PELP1 in GES-1. (B) saRNA for PELP1 increased the proliferation ability of GES-1. (C) saRNA for PELP1 increased the colony formation ability of GES-1. (D) saRNA for PELP1 increased the invasion ability of GES-1. *** p < 0.001.

Article Snippet: Immunohistochemical staining using a polyclonal antibody against PELP1 (A13414, 1:100 for IHC analysis, ABclonal) was performed.

Techniques: Activation Assay, Migration, In Vitro, Western Blot

PELP1 knockdown downregulated c-Src-PI3K-Erk pathway. (A) BIOCARTA analysis of PELP1 modulation of estrogen receptor activity in human pathway. AGS and SNU-1 cells were transfected with PELP1 siRNA or a non-targeting siRNA (control) for 48 h. (B) PELP1 silencing was accompanied by downregulation of c-Src and decreased phospho-Src-Y529 protein as determined by western blot. (C) PELP1 silencing was accompanied by the downregulation of c-Src mRNA, PI3K mRNA and Erk mRNA as determined by quantitative RT-PCR. Values are the mean ± SEM (* p < 0.05, ** p < 0.01, *** p < 0.001).

Journal: Frontiers in Oncology

Article Title: PELP1 Suppression Inhibits Gastric Cancer Through Downregulation of c-Src-PI3K-ERK Pathway

doi: 10.3389/fonc.2019.01423

Figure Lengend Snippet: PELP1 knockdown downregulated c-Src-PI3K-Erk pathway. (A) BIOCARTA analysis of PELP1 modulation of estrogen receptor activity in human pathway. AGS and SNU-1 cells were transfected with PELP1 siRNA or a non-targeting siRNA (control) for 48 h. (B) PELP1 silencing was accompanied by downregulation of c-Src and decreased phospho-Src-Y529 protein as determined by western blot. (C) PELP1 silencing was accompanied by the downregulation of c-Src mRNA, PI3K mRNA and Erk mRNA as determined by quantitative RT-PCR. Values are the mean ± SEM (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Immunohistochemical staining using a polyclonal antibody against PELP1 (A13414, 1:100 for IHC analysis, ABclonal) was performed.

Techniques: Knockdown, Activity Assay, Transfection, Control, Western Blot, Quantitative RT-PCR

Low PELP1 protein expression is a significant prognostic factor favoring good survival in gastric cancer (GC) patients. GC patients were grouped in 2 categories, those having high and low PELP1 gene expression based on the median. Box plots and data derived from ONCOMINE. Bioinformatics result was partly demonstrated by Kanplan-Meier survival analysis. (A) The survival curve analysis showed that there is a obviouly increase in the survival of the patients that have low PELP1 expression. (B) Box plots of gastric cancer grade showed that expression of PELP1 is relatively increased in higher grade. Values are the mean ± SEM. (C) The data from thirty-six GC tissues microarray revealed that patients with decreased PELP1 showed a longer OS ( p < 0.05) (* p < 0.05, ** p < 0.01, *** p < 0.001).

Journal: Frontiers in Oncology

Article Title: PELP1 Suppression Inhibits Gastric Cancer Through Downregulation of c-Src-PI3K-ERK Pathway

doi: 10.3389/fonc.2019.01423

Figure Lengend Snippet: Low PELP1 protein expression is a significant prognostic factor favoring good survival in gastric cancer (GC) patients. GC patients were grouped in 2 categories, those having high and low PELP1 gene expression based on the median. Box plots and data derived from ONCOMINE. Bioinformatics result was partly demonstrated by Kanplan-Meier survival analysis. (A) The survival curve analysis showed that there is a obviouly increase in the survival of the patients that have low PELP1 expression. (B) Box plots of gastric cancer grade showed that expression of PELP1 is relatively increased in higher grade. Values are the mean ± SEM. (C) The data from thirty-six GC tissues microarray revealed that patients with decreased PELP1 showed a longer OS ( p < 0.05) (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: Immunohistochemical staining using a polyclonal antibody against PELP1 (A13414, 1:100 for IHC analysis, ABclonal) was performed.

Techniques: Expressing, Gene Expression, Derivative Assay, Microarray

PELP1 can be targeted by chlorpromazine in GC. (A) Chlorpromazine interacts with PELP1 in molecular docking model. (B) The absorbance (OD) at 450 nm wave-length was reduced in chlorproazine groups in MTT assays dose-dependently. (C1,C2) The colony formation ability of AGS and SNU-1 were inhibited with the increasing of CPZ. Transwell small chamber assay revealed CPZ suppressed the migration and invasion ability of AGS (D1,D2) and SNU-1 cell (D3,D4) . (E) CPZ inhibited the protein expression of PELP1 in AGS and SNU-1. (F) PELP1 activation compromised the CPZ's inhibitory effect on GC AGS cell. Experiments were repeated 3 or 4 times. Values are the mean ± SEM (*** p < 0.001).

Journal: Frontiers in Oncology

Article Title: PELP1 Suppression Inhibits Gastric Cancer Through Downregulation of c-Src-PI3K-ERK Pathway

doi: 10.3389/fonc.2019.01423

Figure Lengend Snippet: PELP1 can be targeted by chlorpromazine in GC. (A) Chlorpromazine interacts with PELP1 in molecular docking model. (B) The absorbance (OD) at 450 nm wave-length was reduced in chlorproazine groups in MTT assays dose-dependently. (C1,C2) The colony formation ability of AGS and SNU-1 were inhibited with the increasing of CPZ. Transwell small chamber assay revealed CPZ suppressed the migration and invasion ability of AGS (D1,D2) and SNU-1 cell (D3,D4) . (E) CPZ inhibited the protein expression of PELP1 in AGS and SNU-1. (F) PELP1 activation compromised the CPZ's inhibitory effect on GC AGS cell. Experiments were repeated 3 or 4 times. Values are the mean ± SEM (*** p < 0.001).

Article Snippet: Immunohistochemical staining using a polyclonal antibody against PELP1 (A13414, 1:100 for IHC analysis, ABclonal) was performed.

Techniques: Boyden Chamber Assay, Migration, Expressing, Activation Assay

Fig. 1 Immunohistochemical staining of PELP1 in TNBC. Positive immunostaining of PELP1 mainly distributed in nuclei of tumor cells, no cytoplasmic staining was found (a, b). Low grade lymph node stage TNBC showed weak PELP1 nuclear expression (a), High grade lymph node stage TNBC showed strong PELP1 nuclear expression (b). PELP1 nuclear staining was absent in negative control (c). Bar = 50 μm.

Journal: BMC cancer

Article Title: Prognostic significance of proline, glutamic acid, leucine rich protein 1 (PELP1) in triple-negative breast cancer: a retrospective study on 129 cases.

doi: 10.1186/s12885-015-1694-y

Figure Lengend Snippet: Fig. 1 Immunohistochemical staining of PELP1 in TNBC. Positive immunostaining of PELP1 mainly distributed in nuclei of tumor cells, no cytoplasmic staining was found (a, b). Low grade lymph node stage TNBC showed weak PELP1 nuclear expression (a), High grade lymph node stage TNBC showed strong PELP1 nuclear expression (b). PELP1 nuclear staining was absent in negative control (c). Bar = 50 μm.

Article Snippet: Polyclonal antibody against PELP1 (Cat. IHC-00013, Bethyl Laboratories, Inc. Montgomery, AL, USA) was applied at an optimized dilution of 1:200 at 4 °C overnight.

Techniques: Immunohistochemical staining, Staining, Immunostaining, Expressing, Negative Control

Fig. 2 Clinicopathological variables and outcomes of patients with TNBC. Kaplan–Meier survival curve showed that TNBC patients with positive lymph node metastasis had significantly reduced DFS (a1) and OS (a2); TNBC patients in stage III and IV also demonstrated significantly reduced DFS (b1) and OS (b2); PELP1 was not associated with DFS or OS in TNBC patients when observed independently, although patients in the high PELP1 group demonstrated a trend of reduced DFS (c1) and OS (c2), compared with those in the low PELP1 group.

Journal: BMC cancer

Article Title: Prognostic significance of proline, glutamic acid, leucine rich protein 1 (PELP1) in triple-negative breast cancer: a retrospective study on 129 cases.

doi: 10.1186/s12885-015-1694-y

Figure Lengend Snippet: Fig. 2 Clinicopathological variables and outcomes of patients with TNBC. Kaplan–Meier survival curve showed that TNBC patients with positive lymph node metastasis had significantly reduced DFS (a1) and OS (a2); TNBC patients in stage III and IV also demonstrated significantly reduced DFS (b1) and OS (b2); PELP1 was not associated with DFS or OS in TNBC patients when observed independently, although patients in the high PELP1 group demonstrated a trend of reduced DFS (c1) and OS (c2), compared with those in the low PELP1 group.

Article Snippet: Polyclonal antibody against PELP1 (Cat. IHC-00013, Bethyl Laboratories, Inc. Montgomery, AL, USA) was applied at an optimized dilution of 1:200 at 4 °C overnight.

Techniques:

Fig. 3 PELP1 protein expression and patients’ outcome in subgroups of TNBC. Kaplan–Meier survival curve showed that, in the tumor size ≤2 cm subgroup, patients with high PELP1 expression had significantly shorter DFS (a1); in the high Ki-67 LI subgroups, patients with high PELP1 expression have significantly shorter DFS (b1) and OS (b2).

Journal: BMC cancer

Article Title: Prognostic significance of proline, glutamic acid, leucine rich protein 1 (PELP1) in triple-negative breast cancer: a retrospective study on 129 cases.

doi: 10.1186/s12885-015-1694-y

Figure Lengend Snippet: Fig. 3 PELP1 protein expression and patients’ outcome in subgroups of TNBC. Kaplan–Meier survival curve showed that, in the tumor size ≤2 cm subgroup, patients with high PELP1 expression had significantly shorter DFS (a1); in the high Ki-67 LI subgroups, patients with high PELP1 expression have significantly shorter DFS (b1) and OS (b2).

Article Snippet: Polyclonal antibody against PELP1 (Cat. IHC-00013, Bethyl Laboratories, Inc. Montgomery, AL, USA) was applied at an optimized dilution of 1:200 at 4 °C overnight.

Techniques: Expressing

Fig. 4 Combining PELP1 status and Ki-67 LI as a prognostic biological marker. Kaplan–Meier survival curve showed that, combination of PELP1 status with Ki-67 status was significantly correlated with DFS (a1) and OS (a2) in patients with TNBC; patients with TNBC in PELP1/Ki-67 double high group had significantly reduced DFS (b1) and OS (b2) compared with others.

Journal: BMC cancer

Article Title: Prognostic significance of proline, glutamic acid, leucine rich protein 1 (PELP1) in triple-negative breast cancer: a retrospective study on 129 cases.

doi: 10.1186/s12885-015-1694-y

Figure Lengend Snippet: Fig. 4 Combining PELP1 status and Ki-67 LI as a prognostic biological marker. Kaplan–Meier survival curve showed that, combination of PELP1 status with Ki-67 status was significantly correlated with DFS (a1) and OS (a2) in patients with TNBC; patients with TNBC in PELP1/Ki-67 double high group had significantly reduced DFS (b1) and OS (b2) compared with others.

Article Snippet: Polyclonal antibody against PELP1 (Cat. IHC-00013, Bethyl Laboratories, Inc. Montgomery, AL, USA) was applied at an optimized dilution of 1:200 at 4 °C overnight.

Techniques: Marker

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